Shearing FACE_PUF DNA for Pico Methyl Seq Library pt.II
Shearing FACE_PUF ACR Sperm Samples Using Q800R3 Sonicator via DNA Shearing Protocol
Continuation of work from Previous Post
After meeting with Hollie from the first round of sonication decided to do more sonications (specific amount to each of the samples)
Specifics for Sonication
| Sample Id | Number of Cycles | Total Time Sonicated (time on) |
|---|---|---|
| ACR_790 | 1 | 15s |
| ACR_185 | 1 | 15s |
| ACR_173 | 5 | 1:15 |
| ACR_754 | 1 | 15s |
| ACR_221 | NA | NA |
| ACR_161 | 1 | 15s |
| ACR_13 | 2 | 30s |
| ACR_778 | 5 | 1:15 |
| ACR_209 | 2 | 30s |
Sample 173 was completely redone and Sample 778 was done for the first time since the concentrations were incorrect
| Sample Id | Avg Concentration (ng/uL) | DNA Input Vol (uL) | Water Input (uL) |
|---|---|---|---|
| ACR_173 | 87.7 | 11.40 | 38.60 |
| ACR_778 | 82.0 | 12.20 | 37.80 |
D5000 TapeStation
Summary of Results
| Sample Id | Number of Cycles | Total Time Sonicated (time on) | BP Length | Plan |
|---|---|---|---|---|
| ACR_790 | 5 | 1:15 | 735 | 1x 15s |
| ACR_185 | 5 | 1:15 | 735 | 1x 15s |
| ACR_173 | 5 | 1:15 | 566 | Done |
| ACR_754 | 5 | 1:15 | 735 | 1x 15s |
| ACR_221 | 4 | 1:00 | 653 | Done |
| ACR_161 | 5 | 1:15 | 685 | 1x 15s |
| ACR_13 | 6 | 1:30 | 960 | 2x 15s |
| ACR_778 | 5 | 1:15 | 728 | 1x 15s |
| ACR_209 | 6 | 1:30 | 606 | Done |
Written on August 20, 2019